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reddot 2 dye  (Biotium)


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    Biotium reddot 2 dye
    Reddot 2 Dye, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reddot+2/RedDot2/pm41750546-102-23-26
    Average 95 stars, based on 164 article reviews
    reddot 2 dye - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Heterogeneous fate choice of genetically modulated adult neural stem cells in gray and white matter of the central nervous system.
    Article Snippet: Blocking was performed with 5–10% biotin-free bovine serum albumin (BSA; in TBS-T) for 30 min at RT, followed by application of the following antibodies (in 5–10% BSA in TBS) and incubation overnight: rabbit anti-NG2 (1:100; MAB5320; Millipore, RRID: AB_11213678; RT), rabbit anti-sex determining region Y-Box 10 (Sox10; 1:100, S1058C002, DCS Immunoline, RRID: AB_2313583; RT), rabbit anti-GFAP (1:10,000; Z0334, DAKO, RRID: AB_10013382; 4 C), goat anti-doublecortin (Dcx; 1:100, sc-8066, Santa Cruz, RRID: AB_2088494; 4 C), rabbit anti-GSTπ (1:4,000; ADI-MSA-101, ENZO, RRID: AB_10615079; 4 C), mouse anti20,30-cyclic-nucleotide 30-phosphodiesterase (CNPase; 1:5,000, 836,402, Biolegend, RRID: AB_2565362; 4 C), rat anti-myelin basic protein (MBP; 1:500, MCA409S, BioRad, RRID: AB_325004; 4 C), rabbit anti-NeuN (1:500; ab177487, Abcam, RRID: AB_2532109; 4 C), mouse anti-myelin oligodendrocytes glycoprotein (MOG; 1:500, MAB5680, Millipore, RRID: AB_1587278; 4 C), and chicken anti-green fluorescent protein/citrine (1:500–2,000; GFP-1020, Aves, RRID: AB_10000240; 4 C or RT). .. Sections were washed two times for 7.5 min in TBS and incubated with the species-appropriate fluorochrome-conjugated secondary antibody (1:500 in PBS) for 30 min at RT: donkey anti-chicken Alexa 488 (703-545-155, Jackson Immuno Research Labs, RRID: AB_2340375), donkey anti-goat Alexa 647 (A-21447, Thermo Fisher Scientific, RRID: AB_2535864), goat anti-chicken Alexa 488 (A-11039, Thermo Fisher Scientific, RRID: AB_2534096), goat anti-rabbit Alexa 405 (A-31556, Thermo Fisher Scientific, RRID: AB_221605), goat anti-mouse Alexa 647 (A-32728, Thermo Fisher Scientific, RRID: AB_2633277), goat anti-rat Alexa 647 (A-21247, Thermo Fisher Scientific, RRID: AB_141778), goat anti-rabbit Alexa 594 (A-11037, Thermo Fisher Scientific, RRID: AB_2534095), and DAPI or RedDot 2 (Biotium, Cat.#: 40061). .. Images were taken using a Zeiss CLSM microscope 510 (CLSM 510, Zeiss, Jena, Germany) and analyzed using the ImageJ BioVoxxel software.

    Article Title: Novel stem cell therapy for cerebral palsy using stem cells from human exfoliated deciduous teeth.
    Article Snippet: .. The tissues were incubated in PBS with 1% Anti-Actin, α-Smooth Muscle-FITC antibody (Sigma-Aldrich, Inc., St. Louis, MO), 1% RedDot 2 (Biotium, Inc., Fremont, CA), and 0.25% Blocker Casein (Thermo Fisher Scientific) for 5 days. ..

    Article Title: Novel stem cell therapy for cerebral palsy using stem cells from human exfoliated deciduous teeth
    Article Snippet: .. The tissues were incubated in PBS with 1% Anti-Actin, α-Smooth Muscle-FITC antibody (Sigma-Aldrich, Inc., St. Louis, MO), 1% RedDot 2 (Biotium, Inc., Fremont, CA), and 0.25% Blocker Casein (Thermo Fisher Scientific) for 5 days. ..

    other:

    Article Title: A Novel Three-Dimensional Imaging System Based on Polysaccharide Staining for Accurate Histopathological Diagnosis of Inflammatory Bowel Diseases
    Article Snippet: If nuclear counterstaining was needed, propidium iodide (10 μg/mL, P21493; Life Technologies, Carlsbad, CA) or RedDot-2 (1:100, 40061-T; Biotium, Fremont, CA) was added to the staining solution.

    Article Title: Combination of the BeWo b30 placental transport model and the embryonic stem cell test to assess the potential developmental toxicity of silver nanoparticles.
    Article Snippet: Cells were washed three times with PBS before incubating the cells for 10min at room temperature with RedDot-2 (1: 200) (Biotium; Fremont, CA, USA) to stain the nuclei.

    Article Title: InVision: An optimized tissue clearing approach for three-dimensional imaging and analysis of intact rodent eyes
    Article Snippet: RedDot 2 , NA , 1:100 , 40,061–1 , Biotium , No.

    Staining:

    Article Title: Amplified genome editing by in vivo editor production
    Article Snippet: Fixed permeabilized cells were washed with 5 w/v% bovine serum albumin (MilliporeSigma, #A9647) in 1× PBS. .. Nuclei were stained with RedDot 2 far-red nuclear stain (Biotium, #40061) for 10 minutes at room temperature. .. Stained cells were washed three times with 5 w/v% bovine serum albumin in 1× PBS before mounting with antifade mounting medium (Vector Laboratories, #VECTH170010).



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    Image Search Results


    A. Competitive ELISA assessing the binding of His-tagged IgGs to immobilized HER2 in the presence or absence of saturating concentrations of non-His-tagged IgGs, detected with an anti-His-HRP antibody. B. Saturation binding curves of trastuzumab and pertuzumab to HER2 on live MCF7 cells. Cells were incubated with antibodies (0-400 nM) for 30 min and detected with an AF647-conjugated goat anti-human secondary antibody via flow cytometry. C. Competitive binding of of AF647-labeled trastuzumab or pertuzumab (0-300 nM) to MCF7 cells pre-saturated with 267 nM of unlabeled trastuzumab and pertuzumab, analyzed by flow cytometry. D. Binding of AF647-conjugated m66 or m75 to MCF7 cells after pre-incubation with 267 nM trastuzumab or pertuzumab. E. Binding of AF647-conjugated rabbit antibodies to MCF7 cells pre-saturated with 267 nM of trastuzumab and pertuzumab, evaluated by flow cytometry. T, trastuzumab; P, pertuzumab. Error bars, SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, T-test.

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    Article Title: Inhibition of HER2 signaling and breast cancer cell growth with a novel antibody targeting HER2 ECD III/IV

    doi: 10.1371/journal.pone.0338127

    Figure Lengend Snippet: A. Competitive ELISA assessing the binding of His-tagged IgGs to immobilized HER2 in the presence or absence of saturating concentrations of non-His-tagged IgGs, detected with an anti-His-HRP antibody. B. Saturation binding curves of trastuzumab and pertuzumab to HER2 on live MCF7 cells. Cells were incubated with antibodies (0-400 nM) for 30 min and detected with an AF647-conjugated goat anti-human secondary antibody via flow cytometry. C. Competitive binding of of AF647-labeled trastuzumab or pertuzumab (0-300 nM) to MCF7 cells pre-saturated with 267 nM of unlabeled trastuzumab and pertuzumab, analyzed by flow cytometry. D. Binding of AF647-conjugated m66 or m75 to MCF7 cells after pre-incubation with 267 nM trastuzumab or pertuzumab. E. Binding of AF647-conjugated rabbit antibodies to MCF7 cells pre-saturated with 267 nM of trastuzumab and pertuzumab, evaluated by flow cytometry. T, trastuzumab; P, pertuzumab. Error bars, SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, T-test.

    Article Snippet: Briefly, each antibody was reacted with 20 molar equivalents of AF647 NHS ester (Biotium, 30–3007) for 2 h at room temperature protected from light.

    Techniques: Competitive ELISA, Binding Assay, Incubation, Flow Cytometry, Labeling